UPF3B antibody

Catalog No. ABIN7120629

This anti-UPF3B antibody is a Rabbit Polyclonal antibody detecting UPF3B in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for UPF3B antibody (ABIN7120629)

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UPF3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-UPF3B Antibody

Purpose: UPF3B antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: UPF3 regulator of nonsense transcripts homolog B(yeast)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IHC:1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for UPF3B

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Alternative Name: UPF3B

Background:

Synonyms: Regulator of nonsense transcripts 3B|Nonsense mRNA reducing factor 3B|Up-frameshift suppressor 3 homolog B (hUpf3B)|Up-frameshift suppressor 3 homolog on chromosome X (hUpf3p-X)|UPF3B|RENT3B|UPF3X

Background: Involved in nonsense-mediated decay(NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex(EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex(including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation, the function is independent of association with UPF2 and components of the EJC core.

Molecular Weight: 58 kDa

Gene ID: 65109

UniProt: Q9BZI7